Effect of Sucrose Concentration on In Vitro Shoot Regeneration in Stevia rebaudiana Bertoni

Vaibhav D. Misal, Ashok M. Chavan

Abstract: Sucrose serves as the primary carbon source in plant tissue culture, influencing cellular growth and development. The present study investigates the effect of varying sucrose concentrations on shoot regeneration in Stevia rebaudiana using nodal explants. Experiments were conducted on Murashige and Skoog (MS) medium supplemented with different sucrose levels. The highest shoot proliferation (19.6?0.452) and maximum shoot length (11.66?0.172 cm) were observed at 40 g/L sucrose concentration. The study highlights the significance of optimizing sucrose levels for enhanced in vitro propagation of Stevia rebaudiana.

Keywords: Stevia rebaudiana, sucrose concentration, micropropagation, plant tissue culture, in vitro regeneration

How to Cite?: Vaibhav D. Misal, Ashok M. Chavan, "Effect of Sucrose Concentration on In Vitro Shoot Regeneration in Stevia rebaudiana Bertoni", Volume 14 Issue 2, February 2025, International Journal of Science and Research (IJSR), Pages: 1383-1384, https://www.ijsr.net/getabstract.php?paperid=SR25222145814, DOI: https://dx.doi.org/10.21275/SR25222145814