Enhanced Antibacterial Activity in Leaf Callus Extracts of Andrographis Paniculata Wall

Hosamani P. A.

Abstract: Antimicrobial activity of leaf and leaf callus extract of Andrographis paniculata Wall., was studied using different solvent like chloroform, acetone, ethanol and water against two - gram (+) ve and two - gram (+) ve bacterial strains like Bacillus subtilis, Staphylococcus aureus, Pseudomonus aeruginosa, and Escherichia coli. The antibacterial activity was determined by disc diffusion method. Out of the four - extract used, acetone and ethanol extracts were found to be highly active in both leaf and leaf extracts. It is also found that the zone of inhibition was much higher in callus extracts, when compared to that of leaf extracts.

Keywords: Andrographis paniculata, callus extract, Pseudomonus aeruginosa Bacillus subtilis, Staphylococcus aureus and Escherichia coli