Investigating the Genetic Impact of Pioglitazone on Insulin Sensitivity in Human Adipocyte Stem Cells
Abstract: Background: The study focuses on Pioglitazone hydrochloride (PH), a PPAR? agonist known for its impact on metabolic diseases. It activates PPAR?, enhancing insulin response in target cells. However, the precise mechanism by which it improves insulin sensitivity remains unclear. Objectives: This research aimed to decipher PH's antidiabetic effects by analyzing key genes (PPAR?, Glut 4, PI3K, TNF?, FASN, IL6) in human adipocytes, vital in diabetes and related issues like atherosclerosis. Methods: Human adipose-derived stem cells were cultured and treated with varying PH concentrations. Cytotoxicity and triglyceride levels were assessed using MTT assays and Oil red O staining. Gene expression (PPAR?, Glut 4, PI3K, TNF?, FASN, IL6) was evaluated through semiquantitative PCR and real-time PCR. Result: At 50 ?M, PH exhibited 70.823% cell viability and significantly reduced lipid accumulation compared to controls. Gene expression analysis revealed heightened PPAR?, GLUT4, PI3K, FASN expression (P
Keywords: Type 2 diabetes mellitus insulin sensitivity, lipid metabolism, adipocyte differentiation, molecular signaling, PPAR? agonist Pioglitazone hydrochloride, semiquantitative PCR and real-time PCR
How to Cite?: Dr. Sunaina Gautam, Dr. M. K Ahmad, Dr. Monisha Banerjee, "Investigating the Genetic Impact of Pioglitazone on Insulin Sensitivity in Human Adipocyte Stem Cells", Volume 14 Issue 1, January 2025, International Journal of Science and Research (IJSR), Pages: 305-311, https://www.ijsr.net/getabstract.php?paperid=SR25105163035, DOI: https://dx.doi.org/10.21275/SR25105163035