Prediction of Site Directed Mutagenesis of Acetylcholinesterase by Using Hotspot Wizard Tool

Kallol Sil, Soumendra Nath Talapatra

Abstract: The present study was predicted mutability position of acetylcholinesterase (PDB ID: 2X8B) by using an online academic tool (HotSpot Wizard, version 3.1). The prediction results were obtained through an output interface in which the functional hotspot of acetylcholinesterase indicated only chain A attached to residues like Glu at 202, Ala at 204 position and Ser at 125 positions, respectively. The pockets were obtained in 1 (catalytic) for all residues in which B - factor values 61.86, 59.91 and 52.35 ?2, respectively. There was predicted higher mutability rate with the score value of 6 in the same chain A. It is concluded that this predictive study is beneficial to detect antidotes related to the specific protein.

Keywords: Acetylcholinesterase, Bioinformatics, Protein engineering, HotSpot Wizard, Mutability position