Prediction of Site Directed Mutagenesis of Acetylcholinesterase by Using Hotspot Wizard Tool

Kallol Sil, Soumendra Nath Talapatra

Abstract: The present study was predicted mutability position of acetylcholinesterase (PDB ID: 2X8B) by using an online academic tool (HotSpot Wizard, version 3.1). The prediction results were obtained through an output interface in which the functional hotspot of acetylcholinesterase indicated only chain A attached to residues like Glu at 202, Ala at 204 position and Ser at 125 positions, respectively. The pockets were obtained in 1 (catalytic) for all residues in which B - factor values 61.86, 59.91 and 52.35 ?2, respectively. There was predicted higher mutability rate with the score value of 6 in the same chain A. It is concluded that this predictive study is beneficial to detect antidotes related to the specific protein.

Keywords: Acetylcholinesterase, Bioinformatics, Protein engineering, HotSpot Wizard, Mutability position

How to Cite?: Kallol Sil, Soumendra Nath Talapatra, "Prediction of Site Directed Mutagenesis of Acetylcholinesterase by Using Hotspot Wizard Tool", Volume 13 Issue 9, September 2024, International Journal of Science and Research (IJSR), Pages: 1597-1600, https://www.ijsr.net/getabstract.php?paperid=SR24926121958, DOI: https://dx.doi.org/10.21275/SR24926121958