Effect of Addition of Phenol on the Quality of Leishman Staining

Vishnu MG, D Neha, Sruthi G

Abstract: This study aims to evaluate the impact of adding phenol to Leishman stain on the quality of peripheral blood smear staining. A modified Leishman stain (MLS) was prepared by adding 35 ?l of liquefied phenol to the conventional Leishman stain (CLS). A comparative analysis was conducted on 20 blood samples, with each sample stained using both MLS and CLS. Results indicate that MLS produces superior staining quality, with better visibility of RBCs, neutrophil, and eosinophil granules, and nuclear details. The modified technique is faster, cost-effective, and yields reliable results, making it a valuable alternative to traditional methods.

Keywords: Effect of phenol, modified Leishman stain, Leishman stain