The Role of Nuclear Activation in the In Vitro Development of Anti - Cancer Mechanisms in Normal Cell Lines

Inas Abd Almajed Rasheed

Abstract: Background: This study investigates the role of nuclear activation in the vitro development of anticancer mechanisms within normal cell lines. By exposing five different normal cell lines to substances like Epidermal Growth Factor, Insulin - like Growth Factor1, Tumor Necrosis Factor - alpha, and Interferon - gamma, we observed a significant changes in cell proliferation, apoptosis, and cytokine release. These Findings suggest that nuclear activation in cancer free cells may trigger anticancer pathways, offering new insights into potential therapeutic strategies for cancer prevention and treatment. Materials and Methods: Five different normal cell lines that were generated from various tissues were employed in this experiment. To induce nuclear activation, cells were exposed to each of the four substances (Epidermal Growth Factor, Insulin - like Growth Factor - 1, Tumor Necrosis Factor - alpha, and Interferon - gamma) for preset amounts of time (24, 48, and 72 hours). We performed a variety of in vitro studies, including cell proliferation, cell death, cell cycle analysis, and cytokine release assays, to investigate possible anti - cancer pathways. The Chi - square test, post - hoc Tukey's test, and two - way ANOVA were all used in the statistical study. Results: According to our research, considerable alterations that occur after nucleus activation might be an indication of anti - cancer processes. With regard to TNF and IFN treatment, we saw a decline in cell proliferation of - 20 percent 3.1 ? 22 percent 3.4, respectively. Following the same treatments, apoptosis rose dramatically by 28 percent 4.3 ? 30 percent 4.5. Additionally, we observed a large increase in cytokine release (25 percent ? 3.8) and a considerable drop in S phase cells (20 percent? 3.2 23 percent ? 3.5, respectively). Conclusions: Our research offers solid proof that nucleus activation in cancer - free cell lines may activate anti - cancer pathways, offering a fresh method for the treatment and prevention of cancer.

Keywords: Nucleus Activation, Normal Cell Lines, AntiCancer Mechanisms, Cell Proliferation, Apoptosis, Cytokine Release

How to Cite?: Inas Abd Almajed Rasheed, "The Role of Nuclear Activation in the In Vitro Development of Anti - Cancer Mechanisms in Normal Cell Lines", Volume 13 Issue 8, August 2024, International Journal of Science and Research (IJSR), Pages: 1620-1627, https://www.ijsr.net/getabstract.php?paperid=SR24821144756, DOI: https://dx.doi.org/10.21275/SR24821144756