Phenotypic and Molecular Characterization of Multidrug - Resistant Uropathogenic Escherichia coli
Abstract: Background: Urinary tract infection (UTI) is the second most common infection after respiratory tract infections in the community. In hospitals, UTI accounts for approximately 35% of all hospital - acquired illnesses. Uropathogenic E. coli (UPEC) accounts for 70%. Increased rates of MDR isolates of E. coli make it a difficult - to - treat pathogen. Materials & Methods: UPEC isolates confirmed by MALDI - TOF were included in the study. The isolates were tested for antibiotic sensitivity to different antimicrobials, and the production of Beta - lactamases viz. ESBL, MBL, and AmpC by phenotypic and genotypic assays. Their clonality was determined by the RAPD. Results: Out of 1150 urinary samples, 122 yielded pathogens, of which 55 were E. coli. Twenty - nine (52.7%) multi - drug resistant isolates were tested for beta - lactamases. All (29) isolates were positive for ESBL, and 6 isolates also harboured MBL genes by PCR. None of the isolates produced AmpC. OXA (n=21) was the commonest followed by TEM and SHV. Among the MBLs, SPM, GIM, IMP, and VIM were detected. Thirteen isolates had Multiple types of beta - lactamase genes. Conclusion: 53 per cent of our E. coli isolates were MDR. All of them produced beta - lactamases. Hence, routine ESBL testing for uropathogens would be useful for all cases of UTI.
Keywords: Beta - lactamase; ESBL; Multidrug resistance; RAPD; uropathogenic Escherichia coli
How to Cite?: Sonowal T, Shariff M, "Phenotypic and Molecular Characterization of Multidrug - Resistant Uropathogenic Escherichia coli", Volume 13 Issue 7, July 2024, International Journal of Science and Research (IJSR), Pages: 605-611, https://www.ijsr.net/getabstract.php?paperid=SR24710130153, DOI: https://dx.doi.org/10.21275/SR24710130153