Biological Study of Synthesised E/Z-4, 5-Dihydro Spiro [3-Phenyl-5-Substituted Phenyl Isoxazole-4, 4' (2',4'-Dihydro-5'-Methyl-2'-Phenyl/ Phenyl Methyl-3'h-Pyrazol-3'-Ones)]

Vipin Kumar Singh, Vijay Pratap Singh, Amrendra Kumar Singh

Abstract: A study on biological activity of synthesized [E-(3a-e) and Z-(3a-e)] spiro isoxazole-pyrazolone derivatives. This examination by single spore isolation and disc diffusion method shows that all compounds have antimicrobial activity. The compounds 3d (E/Z) shows best antibacterial activity against Gram positive bacteria Staphylococcus aureus, Bacillus subtilis, Gram negative bacteria Pseudomonas aeruginosa, Escherichia coli and antifungal activity against Aspergillus niger.

Keywords: Spiro isoxazole-pyrazolones, Antibacterial activity, Inhibition zones, Single spore isolation, Disk diffusion