Assessing the Effectiveness of Xylene-Free Deparaffinization in Hematoxylin and Eosin Staining

Sreeganesh A S, Ramjeela S

Abstract: Xylene is the deparaffinizing agent used in Hematoxylin and Eosin staining. It is expensive, dangerous, and will contaminate the workplace. There's also the disposal issue. Therefore, using a low-cost and environment friendly deparaffinization replacement is highly beneficial. To assess how well xylene-free sections perform in terms of yielding sufficient H and E staining in a straightforward, economical, and faster manner in comparison to traditional H and E sections, forty paraffin blocks were taken into account, a piece was stained using the traditional H and E procedure and another using the H and E method without xylene. Slides were graded according to the following criteria: uniformity, clarity, crispness, nuclear and cytoplasmic staining.

Keywords: Hematoxylin, Eosin, Xylene, Deparaffinization

How to Cite?: Sreeganesh A S, Ramjeela S, "Assessing the Effectiveness of Xylene-Free Deparaffinization in Hematoxylin and Eosin Staining", Volume 13 Issue 5, May 2024, International Journal of Science and Research (IJSR), Pages: 1143-1145, https://www.ijsr.net/getabstract.php?paperid=SR24517233222, DOI: https://dx.doi.org/10.21275/SR24517233222