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Research Paper | Chemical Sciences | India | Volume 13 Issue 2, February 2024

Purification of Crude Ethyl Acetate Extract of M. Spicata Leads to Isolation of Pure Compounds by Different Chrmatographic Methods

Dr. Dnyaneshwar G. Karpe

Abstract: Moullava spicata is a candy corn plant belongs to family Caesalpinacae; it is also called as Wagatea spicata. Crude extracts of this plant shows various prominent biological activities. Reports on presence of various phytoconstituents from M. spicata are available. In present investigation, fresh plant material was collected, shade dried, pulverized and extracted in different solvents with their increasing polarity. Extraction is done in Soxhlet apparatus, solvents are removed under reduced pressure. Dried extracts were screened for TLC and HPLC analysis. Repeated Column chromatography, Preparative Thin Layer Chromatography and Recrystallization techniques are used to isolate pure compound from crude extracts. Characterization of isolated compound was done by spectroscopic methods and compared with authentic sample, it was identified as Quercetin.

Keywords: Moullava spicata, Isolation, Chromatography, Quercetin, Preparative TLC

Edition: Volume 13 Issue 2, February 2024,

Pages: 1195 - 1197