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Research Paper | Biotechnology | India | Volume 13 Issue 1, January 2024

Development of Serodiagnostic Method with the Help of Combination of Immunodominant TB Antigens

Shalmali S. Pendse | Ajitha Rani R

Abstract: Introduction: Tuberculosis (TB) remains one of the world's oldest and most persistent infectious diseases, posing a significant global health challenge. The development of a more efficient and quicker diagnostic method is crucial to stopping the tuberculosis (TB) epidemic. TB infection is known to elicit a robust humoral immune response, making serological tests an attractive approach for diagnosis. In this study, we designed a combination of epitopic peptides derived from various immunodominant TB proteins for detection of antibodies directed against these primary invader sites. Method: Using ELISA, an attempt was made to assess the antibody response to peptides generated from proteins Rv1886c, Rv0934, Rv1926c, Rv1980c and Rv0538 of M. tb in TB patients and healthy individuals. Result: Antibody levels against M. tb peptides were evaluated in 80 TB patients and 150 healthy controls. TB patients showed a stronger immunological response in comparison to healthy individuals and there was a significant difference between the patients and healthy controls (P < 0.000*). Conclusion: Significant statistical differences were observed in the antibody levels against these peptides between PTB patients and healthy individuals in our investigation. These peptides may provide a novel candidate (or candidates) for the early detection of tuberculosis.

Keywords: TB Diagnosis, Immunodominant proteins, ELISA, B cell epitopic peptides, humoral immunity

Edition: Volume 13 Issue 1, January 2024,

Pages: 1542 - 1546