In Vitro Evaluation of Toxin Binder's Binding Capacity in Commercial Broiler Feed

Dr. Dimple Kaparwan, Dr. Nitin Singh

Abstract: This research delves into the intricate assessment of Ran Livtox, a specialized toxin binder, aiming to comprehensively understand its efficacy in alleviating the adverse effects of mycotoxins. Employing a sophisticated in vitro model coupled with the thin - layer chromatography analysis technique, we systematically quantified free mycotoxins, specifically focusing on aflatoxin B1, ochratoxin, and T - 2 toxin (T - 2). The comprehensive evaluation of Ran Livtox (0.2%) extended to individual and combinatory scenarios, with meticulous scrutiny at varying pH levels of 4.5 and 6.5 within the context of poultry diets. The outcomes of our investigation revealed a substantial and statistically significant (p < 0.05) elevation in binding affinity for Aflatoxin (94.71%). Furthermore, the binder exhibited robust binding efficacy for T - 2 (84.28%) and Ochratoxin (93.13%). Intriguingly, this superior binding was notably accentuated at a pH of 6.5 compared to the pH 4.5 environment. This study not only underscores the impressive efficacy of Ran Livtox in binding Aflatoxin but also sheds light on its notable performance concerning T - 2 and Ochratoxin. The pH - dependent variations in binding efficacy underscore the intricate interplay between Ran Livtox and mycotoxins within the complex gastrointestinal environment of poultry. These findings contribute significantly to our understanding of Ran Livtox as a potential solution in combating mycotoxicosis in poultry, laying the groundwork for further exploration and application in real - world scenarios.

Keywords: Ran Livtox, Aflatoxin, Ochratoxin A, T - 2 toxin, binder, in vitro. RAN