Unraveling the History and Mysteries of RNA Sequencing Technology

Amitesh Chakraborty, Rishav Kar

Abstract: RNA sequencing is a technology used extensively in transcriptomics studies. Next - generation sequencing is incorporated in the RNA - seq approach for RNA profiling to deliver unparalleled resolution for analysing and contrasting gene expression patterns. Data pre - processing is one of three main sections of the RNA - sequencing data analysis workflow. An overview of the techniques used in bulk RNA - seq and single - cell analysis is presented here, with an emphasis on alternative splicing and active RNA production study. Removal of adapters, quality checking, trimming, and filtering are essential components of data pre - processing. The data are then post - processed and subjected to a variety of analyses, such as alternative splicing, differential gene expression, and analysis of active synthesis.

Keywords: RNA - Sequencing, Nascent mRNA analysis, Transcriptomic data analysis, Alternative splicing

How to Cite?: Amitesh Chakraborty, Rishav Kar, "Unraveling the History and Mysteries of RNA Sequencing Technology", Volume 13 Issue 1, January 2024, International Journal of Science and Research (IJSR), Pages: 1057-1065, https://www.ijsr.net/getabstract.php?paperid=SR24109000554, DOI: https://dx.doi.org/10.21275/SR24109000554