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Research Paper | Microbiology | India | Volume 12 Issue 9, September 2023 | Popularity: 4.7 / 10

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Isolation of Bacillus subtilis from Dairy Waste and Evaluation of their Protease Kinetics on Fortified Dairy Waste Effluent

Sumit Kumar Dubey, Rashmi Parihar

Abstract: The microbial cells serve asa valuable source of protease due to their diverse metabolic profile and cost - effectiveness. TheBacillus subtilisis widely accepted as a potentalkaline protease producer. These alkaline proteases are widely used as detergent additives, household cleaning agents, partial digestion of proteinaceous food, and waste treatment. The bacterial strains were isolated from dairy waste effluent and among them, the protease - producing B. subtilis was screened. The PPB - 16 (B. subtilis) exhibited the maximum quantitative protease activity of 41 ±0.33 U mL - 1 on Fortified Dairy Waste Effluent (FDWE). The kinetics study of PPB - 16 (B. subtilis) - derived protease revealed bacterial species has also revealedVmax of 8.29 U mg - 1 proteinand Km of 2.37mg casein mL - 1 in FDWE. The prospects include the characterization of protease and their in - silico profiling for the best possible targets that could potentially be used in the food and pharmaceutical sectors. The limitation of the present study is that, as the literature claimed huge diversity in ingredients of dairy waste effluents accordingly protocols for protease production needs to be optimized accordingly.

Keywords: Alkaline Protease, PPB - 16 (Bacillus subtilis), Fortified Dairy Waste Effluent (FDWE), Kinetics, food and pharmaceutical sector

Edition: Volume 12 Issue 9, September 2023

Pages: 2148 - 2151

DOI: https://www.doi.org/10.21275/SR23927174211

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