Measurement of Equine Placental Gonadotropin with the Antiserum Specific to Equine Pituitary Gonadotropin from Pregnant Mare Serum

Abstract: Rabbit antiserum to equine pituitary Luteinizing Hormone (eLH) was generated and characterized in terms of titer, specificity and cross reaction with probable antigens with potential shared epitopes. The rabbit antiserum to equine pituitary Luteinizing Hormone (eLH) is intriguing as it demonstrates its ability to cross - react with other related hormones such as Pregnant Mare Serum Gonadotropin (PMSG) to varying degrees presents an interesting tool for hormone measurement and these hormones share some common epitopes or structural similarities with eLH. While cross - reactivity offers opportunities for multiplexed hormone measurements, it also requires careful consideration during assay development and data interpretation to ensure accurate and specific results. Further, a competitive ELISA based on the rabbit antiserum to eLH was standardized for the measurement of Pregnant Mare Serum Gonadotropin (PMSG) from the PMS. By calibrating this assay, it becomes possible to quantify the amount of PMSG present in an unknown sample based on the degree of competition with the labelled PMSG.

Keywords: PMSG, eLH, FSH, CG, Gonadotropin

Edition: Volume 12 Issue 8, August 2023,

Pages: 724 - 729