Comparative Analysis of Genetic and Phytochemical Variations in in vivo and in vitro Cultures of Trachyspermum roxburghianum Using RAPD and GC-MS Techniques

Sreeranjini K., Thoppil J. E.

Abstract: The in vivo and in vitro plants of Trachyspermum roxburghianum subjected to GC-MS and RAPD studies revealed variations. The essential oil analysis revealed development of new components in in vitro plant. The RAPD studies showed the presence of new bands as well as absence of some bands in the in vitro plant on comparison with the in vivo plant. The present study clearly brings to light that somaclonal variation is associated with tissue culture of T. roxburghianum.

Keywords: Apiaceae, RAPD, Coefficient of similitude, GCMS, Phytochemistry, genetic analysis