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Student Project | Biotechnology | India | Volume 12 Issue 7, July 2023
Juice Sample from Street Side Shop
Misbah Sultana | Prajakta S [4] | Rakshitha K | Sanjeetha S
Abstract: Five crowded juice centres which are present in Peenya (Bangalore) was selected and freshly prepared orange juice sample was collected from each of these places respectively and examined microbiologically. Macroscopic and microscopic features were determined. To identify the morphological features, gram staining was carried out to differentiate between gram positive and negative bacteria. Five biochemical tests were performed i.e. catalase, oxidase, starch hydrolysis, MR and VP. These tests helped in the identification of bacteria. Molecular identification like DNA isolation, polymerase chain reaction, gel elution, Sanger PCR, bioinformatics analysis was done for bacteria characterization. The organisms encountered include Escherichia coli, Vibrio cholerae, Klebsiella pneumoniae, Shigella, Citrobacter. Antibiotic susceptibility test was done using 10 different commonly used antibiotics, Amoxicillin, Azithromycin, Doxycycline, Norfloxacin, Cephalexin, Ciprofloxacin, Cefpodoxime, Cefixime, Amoxicillin + Clavulanate, Metronidazole. The size of zone of inhibition (diameter of areas without bacterial growth) was estimated in mm. It was evident, that the most susceptible antibiotic is Cephalexin with a zone of inhibition of 50mm. The least resistant is Cefpodoxime with 13mm zone of inhibition.
Keywords: Street side shop, Peenya, resistance, susceptibility, antibiotics
Edition: Volume 12 Issue 7, July 2023,
Pages: 1748 - 1752