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Masters Thesis | Dentistry | United Kingdom | Volume 12 Issue 7, July 2023

Biocompatibility of Silicate Based Root Canal Sealers on Human Fibroblast Cells-In Vitro Study based on Cell Viability Assay

Dr. Aswiyaa Muhamed Ilyas

Abstract: Biocompatibility of any root canal sealer plays an important role in determiningthe outcome of the treatment. The recently launched silicate-based root canal sealers claim to have better biocompatibility compared to the regularly used sealers. In vitro study was done to compare the recently launched silicate-based root canal sealers (BioRoot RCS and Totalfill BC) with AH+ the ?Gold standard? sealers used in clinical practice using cell viability assays and immunocytochemistry. Discs prepared from the respective sealers were placed with MM1 cell line (human gingival fibroblast) and WPS9 cell line (human periradicular fibroblast) for seven days to check the biocompatibility of these materials. The remaining live cells were either fixed with cold methanol for immunocytochemistry evaluation or lysed for proyein analysis. Cell viability was evaluated by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-dipheyl tetrazolium bromide (MTT) assay using the material extracts collected at 24, 72 and 168 hours respectively. MTT assay demonstrated AH+ to be the most toxic followed by BioRot RCS and Totalfill BC.

Keywords: Biocompatibility, Tricalcium silicate sealers, fibroblasts, AH+, MTT assay

Edition: Volume 12 Issue 7, July 2023,

Pages: 1842 - 1852