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Research Paper | Microbiology | Indonesia | Volume 12 Issue 6, June 2023

Comparing the Result of Xpert MTB/RIF Assay with iiPCR POCKIT TB in TB Suspect

Ambar Triana | Kristanti Herietrenggi | Abas Suherli

Abstract: One of the strategies of the Indonesian government for eliminating tuberculosis (TB) is the provision of sensitive and specific diagnostic tools to diagnose Mycobacterium tuberculosis. Besides culture examination as the gold standard, molecular - based methods such as Xpert MTB/RIF and isolated isothermal polymerase chain reaction (iiPCR) exist. The purpose of this study was to compare the result of TB examination between Xpert MTB/RIF assay with iiPCR Pockit TB assay in patients with suspected TB at dr. Chasbullah Abdulmadjid Hospital. The study method is analytical observational with a cross - sectional approach using 47 sputum samples. TheXpert MTB/RIF examination results were compared with the iiPCR Pockit. Data were analyzed by cross - tabulation and Kappa. It showed value using Xpert MTB/RIFassay 38 (80.9%) positive results were obtained, and 37 (78, 7%) samples were detected positive for iiPCR Pockit TB. The comparison of results found that there were 3 (6.4%) samples whose results did not match the two methods. Based on Kappa Cohen's statistical analysis, the kappa value of 0.802 means there is a good agreement, and the standard error value of 0.109 with a significant level of 0.000 means there is no statistically significant difference between the Xpert MTB/RIF and iiPCR Pockit TB to detecting Mycobacterium tuberculosis. Thus, iiPCRPockit TB may serve as an alternative method for initial screening and provide a valuable platform for TB detection.

Keywords: iiPCR Pockit, Tuberculosis, Xpert MTB/RIF

Edition: Volume 12 Issue 6, June 2023,

Pages: 2875 - 2878