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Analysis Study Research Paper | Biomedical Sciences | Indonesia | Volume 12 Issue 6, June 2023
Administration of Mangosteen Peel Extract (Garcinia mangostana) Prevented the Decrease of Leydig Cells Numbers, Sertoli Cells Numbers, and Testosterone Hormone Levels in Rats (Rattus norvegicus) Male Wistar Strains Exposed to Cigarette Smoke
Abstract: Introduction: Inhalation of cigarette smoke containing nicotine can cause a decrease in the number of Leydig cells and Sertoli cells. Damage to Leydig cells and Sertoli cells causes a deficiency of testosterone which is one of the signs of aging. Mangosteen peel contains xanthone and tannin compounds which have effects as antioxidants, antibacterial, hypoallergenic, antitumor, antihistamine and anti-inflammatory. This study aimed to prove that administration of mangosteen peel extract (Garcinia mangostana) prevented a decrease in the number of Leydig cells and Sertoli cells, and the level of testosterone in male Wistar rats (Rattus norvegicus) exposed to cigarette smoke. Methods: This was true experimental research using post-test only control group design. The subjects were 36 male Wistar rats aged 12-16 months, weighing 180-200 grams that were randomly divided into 2 groups, namely the control and treatment group. Control group was given 2mldistilled water as placebo and treatment group was given 8mg/200g BW of mangosteen peel extract dissolved in 2 ml distilled water and the rats were then exposed to cigarette smoke 1 hour after administration for 14 days. Blood sample and testes were taken after 14 days to analyze testosterone level and the number of Leydig cells and Sertoli cells. Results: The mean number of Leydig cells in the control group and the treatment group was 4.07 ? 2.19 cells/field of view (FOV) and 7.76 ? 2.28 cells/FOV respectively (p<0.001). The median number of Sertoli cells in the control group and the treatment group was 1.00 cells/FOV and 1.33 cells/FOV respectively (p=0.024). The mean testosterone level in the control group was 2.93 nmol/L, while in the treatment group was 11.25 nmol/L (p<0.001). Conclusion: Administration of mangosteen peel extract (Garcinia mangostana) prevented a decrease in the number of Leydig cells, Sertoli cells and testosterone level in male Wistar rats (Rattus norvegicus) exposed to cigarette smoke.
Keywords: mangosteen peel extract, Leydig cells, Sertoli cells, testosterone, male Wistar rats
Edition: Volume 12 Issue 6, June 2023,
Pages: 2498 - 2503