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Research Paper | Biomedical Sciences | Indonesia | Volume 12 Issue 6, June 2023

Phytochemical Analysis, Antioxidant Activity, and Cytotoxic Activity of Lentinula Edodes (Shiitake Mushroom) Extracts towards Breast Cancer Cell Line T47D

Ade Arsianti

Abstract: Breast cancer is a major threat as it is the number one cancer affecting women. Factors such as genetics, environment, and lifestyle play a role to cause cancer. Humans are constantly exposed to free radicals. An imbalance between free radicals and antioxidants will cause uncontrollable and excessive free radicals that cause cell damage which leads to uncontrollable cell growth. Shiitake mushroom (Lentinula edodes) is one of fungi that has been studied extensively on its health benefit as an antioxidant and anticancer. The study aims to investigate phytochemical constituent, antioxidant activity, and cytotoxicity of Lentinula edodes towards T47D breast cancer cells. Dry powder L. edodes was extracted with n-hexane, ethylacetate, and ethanol sequentially producing L. edodes extracts. Each extract is evaluated for its phytochemical constituents, antioxidant activity by DPPH assay, and cytotoxic activity using MTT assay towards T47D breast cancer cells. Phytochemical screening of L. edodes extracts showed it contains alkaloid, glycoside, flavonoid, and triterpenoid. DPPH assay of L. edodes ethanol extract resulted in active antioxidant activity on DPPH. Based on MTT assay, three L. edodes extracts resulted in very active cytotoxicity towards T47D cells.It ought to be pursued to develop L. edodes as an antioxidant and breast cancer therapeutic.

Keywords: Lentinula edodes, shiitake mushroom, antioxidant, cytotoxicity, T47D cells

Edition: Volume 12 Issue 6, June 2023,

Pages: 2044 - 2049