Analysis of St. John's Wort in Distilled Water by UV - Visible Spectrophotometric method

Dr. Rajeev Kumar Sharma, Rahul Kumar

Abstract: In the present study a simple, precise, sensitive, accurate, selective, sensitive and cost effective UV-visible spectrophotometric method has been developed for analysis and estimation of St. John's wort in distilled water The solution of St. John's wort was scanned over UV-Visible range (up to 630mn) for its wave length of maximum absorbance different calibration standard of St-John's wort were prepared and absorbance was records at?maxat 640nm Beer's Lambert laws is obeyed in calibration curve of concentration Vs absorbance was plotted with concentration range 1-8 ?g/mL. The results of this analysis ware validated statistically and was found to be satisfactory. The developed UV-visible method was successfully applied for the estimation of St. John wort in the routine analytical work.

Keywords: UV, visible spectrometry, St. John's wort validation, Distilled Water, Spectrophotometric Analysis

How to Cite?: Dr. Rajeev Kumar Sharma, Rahul Kumar, "Analysis of St. John's Wort in Distilled Water by UV - Visible Spectrophotometric method", Volume 12 Issue 6, June 2023, International Journal of Science and Research (IJSR), Pages: 63-65, https://www.ijsr.net/getabstract.php?paperid=SR23529153103, DOI: https://dx.doi.org/10.21275/SR23529153103