Analysis of St. John's Wort in Distilled Water by UV - Visible Spectrophotometric method

Dr. Rajeev Kumar Sharma, Rahul Kumar

Abstract: In the present study a simple, precise, sensitive, accurate, selective, sensitive and cost effective UV-visible spectrophotometric method has been developed for analysis and estimation of St. John's wort in distilled water The solution of St. John's wort was scanned over UV-Visible range (up to 630mn) for its wave length of maximum absorbance different calibration standard of St-John's wort were prepared and absorbance was records at?maxat 640nm Beer's Lambert laws is obeyed in calibration curve of concentration Vs absorbance was plotted with concentration range 1-8 ?g/mL. The results of this analysis ware validated statistically and was found to be satisfactory. The developed UV-visible method was successfully applied for the estimation of St. John wort in the routine analytical work.

Keywords: UV, visible spectrometry, St. John's wort validation, Distilled Water, Spectrophotometric Analysis