Effects of Low Level Laser of 650nm on Vialbility of Aggregatibacter Actinomycetemcomitans with and without Photosensitisers: An Invitro Study

Dr. M M Dayakar | Dr. Apoorva .G | Dr. Devipriya B | Dr. Nihal Abbas

Abstract: Aggressive periodontitis (Grade C periodontitis) is significantly correlated with Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). The aim of this investigation was to compare the effect of low level laser of 650nm on vialbility of aggregatibacter actinomycetemcomitans with and without photosensitisers. Thirty samples of bacterial suspensions (200?l) were prepared and divided into six groups: laser group (low level laser with wavelength of 6oonm, and irradiation time of 30 s), Methylene blue + laser group (after pre - irradiation time of 10 min, laser was irradiated), and Toluidine blue O + laser group (after preirradiation time of 10 min, LED was irradiated), Methylene blue group (0.1 %), Toluidine blue O (TBO) group (0.1 mg/ mL) and control group (no treatment), Then, 100 ?L of each sample was cultured in blood agar plates and incubated for 72 h in microaerophilic atmosphere for colony counting. Application of Methylene blue + laser and Toluidine blue O + laser group resulted in a significant decrease in the concentration of A. actinomycetemcomitans (P values< 0.005) Within the limits of this study, it can be concluded that photodynamic inactivation using laser and Photosensitizers like Methylene blue and Toulidine blue was more effective than photosensitizers and laser irradiation alone in eradication of A. actinomycetemcomitans.

Keywords: Methylene blue, Toluidine blue O, Low level laser, Aggregatibacter actinomycetemcomitan

Edition: Volume 12 Issue 5, May 2023,

Pages: 2435 - 2437