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Research Paper | Pathology | India | Volume 12 Issue 3, March 2023
Comparison of Conventional Papanicolaou Stain with Enviro Papanicolaou Stain in Buccal Smear
Abstract: The traditional screening method for cervical cancer is the PAP smear. As practiced conventionally PAP stain is expensive utilizing a considerable amount of alcohol and consumes a lot of time. This study is to find out a standard technique which is superior or comparable to conventional PAP staining, rapid and cost effective with minimal alcohol use. This study aimed to compare Conventional Papanicolaou stain and Enviro PAP stain in buccal smear. We collect two buccal smears from 500persons and the smears were stained with PAP and Enviro PAP. Then examine the slides under microscope to assess its utility depending on quality of staining assessed by nuclear characteristics, cytoplasmic details, cell morphology, artifact and background. The Enviro PAP staining quality is closely related to Conventional PAP staining Quality. Enviro PAP procedure has minimum alcohol use and technician friendly protocol that does not compromise on staining quality and diagnostic standards. This staining procedure is simple and environmental friendly. Using Enviro PAP can substantially reduce a laboratory?s annual staining reagent purchase and Xylene disposal cost. It can be easily adopted as a suitable alternative to the expensive and time consuming standard protocol for mass screening of cervical cancer in limited resource settings.
Keywords: Papanicolaou Stain, PAP, Ultra Fast Papanicolaou Stain, UFP, Modified Ultrafast Papanicolaou Stain, MUFP, Eosin Azure, EA, Orange GELB, OG
Edition: Volume 12 Issue 3, March 2023,
Pages: 1550 - 1552