Production and Purification of Nattokinase from B. Subtilis

Shivani Tiwari

Abstract: Nattokinase, novel fibrinolytic enzymes which are isolated from Bacillus subtilis isolated from soil samples. The fibrinolytic enzyme Nattokinasewas purified from supernatant of Bacillus subtilis, showed thermophilic, hydrophilic, and strong fibrinolytic activity. The maximum production was carried out in the presence of maltose as carbon source and peptone as nitrogen source. Optimum temperature and pH of Nattokinase, was 37?C and 7 respectively. The fibrinolytic activity of Nattokinase was 576.73 U, respectively.

Keywords: Anticoagulant Activity, Submerge Fermentation, Fibrinolytic Enzyme Activity, Haemolytic Activity