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India | Enzymology Science | Volume 12 Issue 2, February 2023 | Pages: 531 - 534
Production and Purification of Nattokinase from B. Subtilis
Abstract: Nattokinase, novel fibrinolytic enzymes which are isolated from Bacillus subtilis isolated from soil samples. The fibrinolytic enzyme Nattokinasewas purified from supernatant of Bacillus subtilis, showed thermophilic, hydrophilic, and strong fibrinolytic activity. The maximum production was carried out in the presence of maltose as carbon source and peptone as nitrogen source. Optimum temperature and pH of Nattokinase, was 37?C and 7 respectively. The fibrinolytic activity of Nattokinase was 576.73 U, respectively.
Keywords: Anticoagulant Activity, Submerge Fermentation, Fibrinolytic Enzyme Activity, Haemolytic Activity
How to Cite?: Shivani Tiwari, "Production and Purification of Nattokinase from B. Subtilis", Volume 12 Issue 2, February 2023, International Journal of Science and Research (IJSR), Pages: 531-534, https://www.ijsr.net/getabstract.php?paperid=SR23205143811, DOI: https://dx.doi.org/10.21275/SR23205143811