Downloads: 4 | Views: 98 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Analysis Study Research Paper | Medical Microbiology | India | Volume 12 Issue 10, October 2023

Sputum-Spotted Solid Matrix Designed to Release Diagnostic-Grade Mycobacterium tuberculosis DNA Demonstrate Optimal Biocontainment Property

Krishna H. Goyani | Pratap N. Mukhopadhyaya

Abstract: Background: Biosafety quotient of a reagent-coated cellulose matrix designed to release Mycobacterium tuberculosis (Mtb) DNA from sputum deposited onto it was determined. Method: Thirty-seven sputum samples infected with Mtb and 5 sputum samples from healthy individuals were processed and spotted onto a chemically coated cellulose matrix named the TBSend cards. Live Mtb bacilli were attempted for rescue from the spotted TBSend cards by washing them in phosphate buffer saline under mild shaking conditions. No live Mtb bacilli could be detected by the BACTEC? MGIT 960? TB System from both Mtb infected and Mtb non-infected sample-spotted TBSend cards when they were washed at two different time-points namely, 1 minute and 15 hours from the time of spotting of the cards with processed sputum. Result: The study consolidated published findings that chaotropic agents, which is an active component of the TBSend card module, have cent per cent bactericidal properties with regard to Mtb. Conclusion: Mtb infected sputum mixed with a biological cell-lysing solution and spotted onto a cellulose matrix coated with chaotropic salts effectively eliminates all live pathogen and can be used as a bio-safe Tb sputum transportation, storage and DNA-release device for Mtb NAAT.

Keywords: TBSend card, M. tuberculosis, Biosafety, Biocontainment

Edition: Volume 12 Issue 10, October 2023,

Pages: 1082 - 1088