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Research Paper | Biological Sciences | Ivory Coast | Volume 11 Issue 9, September 2022 | Popularity: 4.6 / 10
Preservation Solution for Liver Tissue Samples from the Wistar Strain of Rattus Norvegicus in Cote d'Ivoire
Ambroise Kouame Kintossou, Lydie Nina Amon, Mireille Dosso
Abstract: Tissue samples must be frozen in liquid nitrogen for cell biology analysis. In C?te d'Ivoire, tissue samples are mainly transported in tanks containing liquid nitrogen. However, liquid nitrogen is a difficult and dangerous product to handle and represents a significant investment. Faced with this problem, it is necessary to prepare a preservation solution in order to determine its capacity to preserve the cellular characteristics of the tissues. A preservation solution called KKA solution was prepared. Five Wistar rats (laboratory rats) were used as experimental animals. These healthy rats were sacrificed, followed by the removal of liver organs. Each rat liver organ was cut into small pieces corresponding to 10 mg. The liver tissue samples were separated into groups to assess cell viability. Proportion tests were used for comparison of the sample groups. The KKA solution preserves more than 80% of liver cells for 24 hours at +4?C. KKA solution protects the cellular characteristics of healthy rat liver tissue for 24 hours at +4?C.
Keywords: Cell viability, preservation solution, liver tissue
Edition: Volume 11 Issue 9, September 2022
Pages: 853 - 857
DOI: https://www.doi.org/10.21275/SR22731180302
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