Comparative Studies on the Enhanced Production of Heparinase by Physical and Chemical Mutagen

Gaurav Upadhyay

Abstract: This experiment was deigned check the comparative studies on the enhanced production of heparinase by physical and chemical mutagen. Heparin is a combined sulfated polysaccharide that has certain characteristics and dispersions. It was initially discovered in the liver and was thus called heparin. In this experiment the growth of heparin was seen under the adverse physical conditions whereas under the chemical conditions heparin couldn?t survive. Physical condition of heparin was kept under UV radiations for different time duration i.e. 2min, 4min and 6min. likewise under chemical condition heparin was added with EtBr i.e. 1?l, 2?l and 3?l. After that in purification the sample collected from my ultraviolet light plate were processed in down streaming. In downstream processing the sample in dialysis bag which was submerged in Tris HCl. By this process the salt content in the bag was excreted out which is called salt precipitation. Finally, Observed the optical density of the sample at 620 nanometre and I found the OD 0.23 on spectrophotometer.

Keywords: Heparinase, UV radiation, EtBr mutation, Down streaming, Optical density

Edition: Volume 11 Issue 5, May 2022,

Pages: 1443 - 1449