International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Research Paper | Pharmacy | India | Volume 11 Issue 5, May 2022


A New Simple, Specific and Validated FT-IR Spectroscopic Method for the Estimation of Sudan Dye Adulteration in Chilli Powder

Gokul J Siddharth | Fathima A. M. | Mohammed Shaheer | Aleyamma Joseph | Saida Sherin | Sheeja Velayudhankutty


Abstract: A simple, rapid, specific and validated method for estimation of Sudan dye II in chilli powder was developed using FTIR. This study was carried out to detect adulterants in chilli powder. Detection test of adulterants which include qualitative and quantitative estimation of Sudan II (carcinogenic dye) in red chili powder by FTIR. For present study twelve chilli powder samples were collected from various localities of Palakkad and analysed. The study includes a detailed investigation. Sudan dye-II and chilli powder sample pellets were prepared by pressed pellet technique. FTIR spectrum obtained with the assistance of the IR solution software, quantitative analysis is carried out in the region 2854cm-1 for ring C-H Stretching and 2362cm-1 related to N=N. Linearity range was 0.015-0.035mg. Precision was calculated and low %RSD values were found. LOD and LOQ were in the range of 0.0017mg and 0.0054mg. Marketed samples showed the presence of Sudan dye-II whereas homemade sample showed absence of Sudan dye-II. Thus this technique is useful for quality control and detection of powder spices where label free and non branded spices are commonly sold in markets.


Keywords: Sudan Dye II, Chilli powder, FTIR, Precision, Robustness


Edition: Volume 11 Issue 5, May 2022,


Pages: 1425 - 1430


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