Downloads: 1 | Views: 193 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1
Research Paper | Biotechnology | Indonesia | Volume 11 Issue 12, December 2022
Analysis of Sulforafan Content in Hybrid Broccoli Supplies in MS Media Delivered NAA and BAP and Methionine in Vitro
Wenny Tilaar [5] | Arthur Pinaria | Nelson Nainggolan [6] | Jeany Polii Mandang [2] | Dingse Pandiangan [13]
Abstract: Broccoli is a vegetable plant that contains sulforaphane as an antioxidant. These compounds are useful for curing cancer. Therefore, research has been carried out on the analysis of sulforaphane content in hybrid broccoli sprouts. The purpose of this research : 1. to determine the sulforaphane content of hybrid Broccoli sprouts growing on MS media 2. To determine the difference in sulforaphane content of hybrid broccoli sprouts grown on media that was given a combination of NAA; BAP and methionine The design used is factorial in RAL with 3 factors, namely A. NAA 0; 0.5 ; 1 ppm B. BAP 0 ; 1 ; 2 ppm and C. methionine 0 g; 25 g and 50 g. Each treatment was repeated 3 times. Variables observed were sprout height, sprout weight and sulforaphane content. The data were analyzed by analysis of variance and continued with the 5% BNT test. The results of the analysis of variance showed that there was an interaction between the treatment of NAA, BAP and Methionine and had a significant effect on the height of hybrid broccoli sprouts. Furthermore, the results showed that the highest sprouts were found in the N0B0M0 treatment or without NAA, BAP and Methionine treatment, which was 10.60 cm. While the lowest in the combination treatment N0.5B0M50 and N0.5B2M50 is 0.80 cm. The higher the concentration of NAA, BAP and Methionine can suppress the increase in shoot height of the hybrid broccoli sprouts. Furthermore, the results of the analysis of variance showed that there was no interaction between NAA, BAP and Methionine on the weight of sprouts but there was an interaction between BAP and methionine in a significant effect on the weight of sprouts. The results of the analysis of variance showed that there was an interaction between NAA, BAP and methionine on sulforaphane biosynthesis. The NoB1M25 combination had the highest sulforaphane content compared to the other treatments and was significantly different from the other treatments.
Keywords: Hybrid Broccoli Sprouts, NAA, BAP, Methionine, Sulforaphane Biosynthesis
Edition: Volume 11 Issue 12, December 2022,
Pages: 1235 - 1239