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Research Paper | Pharmaceutical Science | India | Volume 11 Issue 11, November 2022

Novel HPTLC Method for Estimation of Fluocinonide

Pooja Kumari Jaiswal | Dr Vandana Jain

Abstract: A novel high-performance thin-layer chromatographic (HPTLC) method was developed and validated for estimation of fluocinonide. The HPTLC separation was achieved on a precoated silica gel 60F254 aluminum sheet as a stationary phase using mixture of chloroform-methanol (19: 1 v/v) as mobile phase. The detection of spots was carried out at 245nm. The method was validated in terms of specificity, linearity, accuracy, precision, repeatability, and robustnessas per the International Conference on Harmonization guidelines. The calibration curve was found to be linear between 100-500 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, sensitive and selective for the analysis of fluocinonide. The method was successfully employed for the estimation of fluocinonide.

Keywords: Estimation, analysis, Fluocinonide, Development, validation

Edition: Volume 11 Issue 11, November 2022,

Pages: 302 - 305