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Research Paper | Biomedical Sciences | Indonesia | Volume 10 Issue 8, August 2021
Administration of Myrmecodia pendans Ethanol Extract Cream Prevented Elevation of Keratinocyte Apoptosis and Number of Macrophages in Male Wistar Rats (Rattus norvegicus) Exposed to Ultraviolet B (UVB)
Diana Utami | Wimpie Pangkahila [22] | I Gusti Made Aman [5]
Abstract: Background: Aging is a natural process that is influenced by several factors, one of which is sunlight/ultraviolet (UV). Continuous exposure to UV radiation causes changes in the structure and function of the skin, ranging from sunburn, which is the apoptosis of keratinocyte cells; to increasing the formation of acute inflammatory cells such as macrophages. This study aimed to prove that administration of M. pendans 40% ethanol extract cream prevented elevation of keratinocyte apoptosis and number of macrophages in male Wistar rats (Rattus norvegicus) exposed to Ultraviolet B (UVB). Methods: This study was an animal experimental study using a randomized post - test only control group design. The subjects were rats (Rattus norvegicus), Wistar strain, male, 2 - 3 months old, 180 - 200 grams body weight, and healthy. The number of samples used was 36, divided into 2 groups (n = 18). The control group was exposed to UVB and treated with placebo cream, the treatment group was exposed to UVB and treated with M. pendans 40% ethanol extract cream. After 24 hours of exposure, the rats were anesthetized and a biopsy of the back skin tissue was performed to examine the apoptotic number of keratinocyte cells and the number of macrophage cells using histological methods. Results: The mean apoptotic number of keratinocyte cells in the control group was 25.1 ? 3.81 cells/field of view, while in the treatment group was 15.0 ? 3.10 cells/field of view (p <0.001). The mean number of macrophage cells in the control group was 3.73 ? 1.14 cells/field of view, while in the treatment group was 2.26 ? 1.09 cells/field of view (p <0.001). Conclusion: The study presented herein proved that administration of M. pendans 40% ethanol extract cream prevented elevation of keratinocyte apoptosis and number of macrophages in male Wistar rats (Rattus norvegicus) exposed to Ultraviolet B (UVB).
Keywords: M. pendans 40% ethanol extract cream, apoptosis of keratinocyte, macrophages, Ultraviolet B, UVB
Edition: Volume 10 Issue 8, August 2021,
Pages: 852 - 856