Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques

Ihsan Edan Alsaimary, Falih Hmood Mezban

Abstract: This study aimed to detect IL 4 and IL 17 by conventional and RT PCR. DNA-based techniques have been further simplified benefiting from the introduction of PCR the result of this estimation revealed that the amplified DNA (PCR product) was 710 bp for IL-4 (50 voltage for 1 hour) and the presence of IL-4 was 85 % from total samples, (PCR product) was 344 bp for IL-17 on agarose gel (1 %) The presence of IL-17 was 70 % from total samples. RT PCR IL-4 show peak between 84.5 85.2 and IL-17 peak between 86.5-87.5.

Keywords: conventional and Real Time q-PCR techniques, human bronchial asthma, interleukins

How to Cite?: Ihsan Edan Alsaimary, Falih Hmood Mezban, "Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques", Volume 10 Issue 2, February 2021, International Journal of Science and Research (IJSR), Pages: 165-169, https://www.ijsr.net/getabstract.php?paperid=SR21119160619, DOI: https://dx.doi.org/10.21275/SR21119160619