Downloads: 89 | Views: 154

Research Paper | Pharmacy | Indonesia | Volume 9 Issue 7, July 2020

Phytochemical Screening, Total Phenolics, Total Flavonoids and Antioxidant Activity of Talas Steam Ethanol Extract (Colocasia esculenta (L.) Schott)

Oktariani Pramiastuti | Desi Sri Rejeki | Rani Hidayatun

Abstract: Talas stems (Colocasia esculenta (L. ) Schott) are known to have antioxidant activity. Extraction of talas stems used maceration method with 96 % ethanol. Determination of total phenolic content used the Folin-Ciocalteu method with a gallic acid comparison compound. Whereas the colorimetric method was used to determine total flavonoid content with 10 % AlCl3 reagent and 5 % acetic acid. The antioxidant activity test applied DPPH (1, 1-diphenyl-2-picrylhydrazyl) method with a quercetin comparison compound. The series of ethanol extract of talas stem were 20, 40, 60, 80 and 100 ppm. The comparison of quercetin was 2, 4, 6, 8 and 10 ppm. The results of total phenolic levels obtained were 78.98 mg GAE / g extract, total flavonoid levels were 97.38 mg QE / g extract and IC50 results of talas stem extract were 74.75 ppm, quercetin was 5.96 ppm. Based on the level of antioxidant strength, quercetin had very strong antioxidant activity (IC50 <50 ppm), because quercetin was a pure compound. While talas stem extract had strong antioxidant activity (IC50 50-100 ppm).

Keywords: Talas, Total phenolic, Total flavonoids, Antioxidants

Edition: Volume 9 Issue 7, July 2020,

Pages: 256 - 259