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Research Paper | Chemistry | Volume 9 Issue 10, October 2020 | Pages: 1072 - 1076 | India
Purification and Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Telangana Region
Abstract: Polyphenol oxidase (PPO) from Zyzyphus spina-christi from Telangana region was extracted and purified by (NH4) 2SO4 precipitation, ion-exchange chromatography and gel filtration chromatography. The biochemical characteristics of reveals that this PPO has higher affinity towards catechol (Km = 12.2mM and Vmax = 17, 200 U/ml min-1) at an optimum pH of 5.75. The enzyme has an optimum temperature of 37.50C and was relatively stable up to 480C for a period of 60 minutes with almost 78 % of activity remaining. Among the various polyphenol oxidase inhibitors tested, the most effective inhibitor for the enzyme with 10mM catechol as substrate was ascorbic acid.
Keywords: Polyphenol Oxidase, Zyzyphus spina-christi, Telangana, Catechol, inhibitor, Optimum
How to Cite?: T R Satyakeerthy, T R Shanthi, Raju Bolla, Lini Nirmala, "Purification and Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Telangana Region", Volume 9 Issue 10, October 2020, International Journal of Science and Research (IJSR), Pages: 1072-1076, https://www.ijsr.net/getabstract.php?paperid=SR201007131037, DOI: https://dx.doi.org/10.21275/SR201007131037