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Research Paper | Biochemical Engineering | India | Volume 3 Issue 11, November 2014

Comparative Effect of Saponin and Aqueous Saponin Extracts of Lycopersiconesculentum in Diabetic Blood

Mita Dutta | Yashodhara Verma ^[3] | Deepshikha Kushwaha | Sushma ^[71]

Abstract: The aim of this study was to evaluate the effect of fresh aqueous extract, lyophilised extract and purified sample of Lycopersiconesculentum on normal and diabetic blood samples. Some of the physicochemical properties as well as antioxidant properties of human blood were studied. Firstly the fresh aqueous extracts of tomato plants were prepared and these extracts were lyophilized.0.2 ml of blood was added to 0.2 ml of different concentrations of each extract in Mcllvaines buffer, and then incubated. The absorbance of the samples was determined by UV spectrophotometer. Among the studied extracts, normal blood showed the highest haemolytic effect when treated with fresh tomato extract. The values of emulsification index (E24) and foam stability index (Fh) for each extract signify the surface activity. Diabetic blood showed more SOD activity than normal blood. Maximum catalase activity was found in diabetic blood containing fresh aqueous extract compared to normal blood. The results obtained showed that purified sample was more effective than fresh aqueous extracts and lyophilised extracts. It was found that saponin has more positive effect on diabetic blood and thus helps in maintaining hypoglycemic conditions.

Keywords: Catalase, Emulsification index E24, Foam stability index Fh, Lycopersiconesculentum, Lyophilised extract, Osmotic fragility, SOD

Edition: Volume 3 Issue 11, November 2014,

Pages: 3030 - 3032