Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)

Sudharani M, Rao PS, Subba Rao LV

Abstract: The present investigation was undertaken to identify specific marker associated with the maize hybrid DMH-117 and its parental lines, hybrid purity testing as well as identify the off-types from the seed lot. The hybrid DHM-117 could be clearly identified by using the SSR loci Umc1600 based on the banding pattern resolved on agarose gel (3 %). The complementary banding pattern of the male and female parents made a way to identify the hybrid. The SSR loci Umc1600 amplified a specific allele size of 180bp in seed parent (BML-6) and 195bp was specific to pollen parent BML-7. These two bands of allele size 180bp and 195bp were found in hybrid DHM-117, there by confirming that this hybrid was produced from the cross combination of BML-6 and BML-7.

Keywords: Genetic purity, Maize hybrids, SSR markers

How to Cite?: Sudharani M, Rao PS, Subba Rao LV, "Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)", Volume 3 Issue 10, October 2014, International Journal of Science and Research (IJSR), Pages: 92-98, https://www.ijsr.net/getabstract.php?paperid=OCT144, DOI: https://dx.doi.org/10.21275/OCT144