International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 109 | Views: 207

Research Paper | Genetics Science | India | Volume 3 Issue 10, October 2014


Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)

Sudharani M [2] | Rao PS [2] | Subba Rao LV


Abstract: The present investigation was undertaken to identify specific marker associated with the maize hybrid DMH-117 and its parental lines, hybrid purity testing as well as identify the off-types from the seed lot. The hybrid DHM-117 could be clearly identified by using the SSR loci Umc1600 based on the banding pattern resolved on agarose gel (3 %). The complementary banding pattern of the male and female parents made a way to identify the hybrid. The SSR loci Umc1600 amplified a specific allele size of 180bp in seed parent (BML-6) and 195bp was specific to pollen parent BML-7. These two bands of allele size 180bp and 195bp were found in hybrid DHM-117, there by confirming that this hybrid was produced from the cross combination of BML-6 and BML-7.


Keywords: Genetic purity, Maize hybrids, SSR markers


Edition: Volume 3 Issue 10, October 2014,


Pages: 92 - 98


How to Download this Article?

Type Your Valid Email Address below to Receive the Article PDF Link


Verification Code will appear in 2 Seconds ... Wait

Top