Shoot Regeneration from Leaf Derived Callus of Coleus forskohlii Briq
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Research Paper | Botany | India | Volume 3 Issue 11, November 2014 | Popularity: 6.3 / 10


     

Shoot Regeneration from Leaf Derived Callus of Coleus forskohlii Briq

Poornima Atulkar, Ritu Thakur, Pratibha Singh


Abstract: Coleus forskohlii Briq. a valuable medicinal important plant, member of the family lamiaceae was investigated for callusing and shoot induction of shoot explants. Leaf was studied using various growth harmones. Auxins (2, 4-D) alone and in combination with cytokinines (BAP) induced green colour, light green colour, cremish, green fragile, brown colour was formed. Best Shoot induction was achieved from the callus using BAP (0.5 mg/l) and NAA (0.1 mg/l) after 2 weeks of inoculation. Where highest no. of shoot induced (3.00 0.28) and with maximum frequency (80 0.56) was regenerated


Keywords: Coleus forskohlii, callusing, in vitro shoot induction, Murashige and Skoogs MS media


Edition: Volume 3 Issue 11, November 2014


Pages: 2662 - 2664



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Poornima Atulkar, Ritu Thakur, Pratibha Singh, "Shoot Regeneration from Leaf Derived Callus of Coleus forskohlii Briq", International Journal of Science and Research (IJSR), Volume 3 Issue 11, November 2014, pp. 2662-2664, https://www.ijsr.net/getabstract.php?paperid=OCT141121, DOI: https://www.doi.org/10.21275/OCT141121

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