Shoot Regeneration from Leaf Derived Callus of Coleus forskohlii Briq

Poornima Atulkar, Ritu Thakur, Pratibha Singh

Abstract: Coleus forskohlii Briq. a valuable medicinal important plant, member of the family lamiaceae was investigated for callusing and shoot induction of shoot explants. Leaf was studied using various growth harmones. Auxins (2, 4-D) alone and in combination with cytokinines (BAP) induced green colour, light green colour, cremish, green fragile, brown colour was formed. Best Shoot induction was achieved from the callus using BAP (0.5 mg/l) and NAA (0.1 mg/l) after 2 weeks of inoculation. Where highest no. of shoot induced (3.00 0.28) and with maximum frequency (80 0.56) was regenerated

Keywords: Coleus forskohlii, callusing, in vitro shoot induction, Murashige and Skoogs MS media