Detection of Mitochondrial DNA T16189C Polymorphism

Nihad A.M. Al-Rashedi, Emad Uldeen Hateem

Abstract: This study can be observed as polymorphic nucleotide where T to C transition was observed at the 16189 nucleotide position in mitochondrial DNA. The aim of this study was to confirm to the mtDNA polymorphic nucleotide which may be useful in the forensic genetics and clinical applications. The 100 blood samples of Iraqi population were collected for mitochondrial DNA extraction and evaluated for mtDNA 16189 variant by PCR-RFLP technique. Then, digested fragments were ready to direct DNA sequencing. The results showed that detection of transition T to C at the position 16189 in mitochondrial DNA by PCR-RFLP and direct DNA sequencing. The frequency of transitional polymorphic nucleotides at positions 16189 were 0.16 observed highest. It is transition generates heteroplasmic length tract of polycytosine which has advantage in mitochondrial DNA fingerprinting and index of different metabolic disorders.

Keywords: mtDNA polymorphism, T16189C, PCR-RFLP, DNA Sequencing