Activated Sludge: Decolorization and Degradation of Textile Dye Reactive Orange HE2R

Pratibha S. Patil

Abstract: At lab scale develop an activated sludge and decolorize three tested textile dyes Reactive Red 198, Reactive Orange HE2R and Navy Blue HE2R 13, 17 and 8 % respectively within 120 h. In presence of Glucose (51 %) and yeast extract (98 %) decolorization of Reactive Orange HE2R dye observed within 24 h. The significantly involved laccase and NADH-DCIP reductase in dye decolorization. The analytical studies like UV-Vis spectroscopy, FTIR and HPLC confirmed the degradation of Reactive Orange HE2R. GC-MS data has been proposed the probable dye degraded pathway and metabolites formed identified as 2-amino butane 4-sulpho aniline and sulphoaniline.

Keywords: Activated sludge, Reactive Orange HE2R, Biodegradation, Enzyme and GCMS