SSR Molecular Marker and Analysis Report of Variety of Chicken for their Varying Weight

Ahmed Kareem Alatafi, Kondapalli Kasturi

Abstract: Microsatellites are easy to genotype and densely distributed throughout eukaryotic genomes, making them the preferred genetic marker for high resolution genetic mapping. The use of DNA marker technology in poultry as a strains identification has progressed rapidly during the last decade. In our case, Simple Sequence Repeats (SSR) analysis for 56 individuals male and female belonging to fast white broiler and Rainbow rooster multicolored dual purpose birds (fast white female ( A), fast white male (B), Rainbow rooster female (C) and Rainbow rooster male (D) using four primers. A total of 480 bands in different loci is generated, range of molecular weight start from 97-2708.

Keywords: Microsatellites, Germ Plasm, Miniprep, Genetic mapping, Dendrogram