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Research Paper | Biochemistry Science | Indonesia | Volume 4 Issue 12, December 2015
Effects of Tender Coconut Water on Antioxidant Enzymatic Superoxida Dismutase (SOD), CATALASE (CAT), Glutathione Peroxidase (GPx) and Lipid Peroxidation In Mercury Exposure Workers
Siti Thomas Zulaikhah [2] | Anies | Ari Suwondo [4] | Santosa [10]
Abstract: Background The traditional gold miners use mercury to process gold. Continuous exposure to mercury will trigger the formation of Reactive Oxygen Species (ROS), increase lipid peroxidation, cause oxidative stress on cells or tissues, inhibit the enzyme, cause cell damage, damage to the structure of proteins, DNA damage, interfere with the body's antioxidant metabolism particularly superoxide dismutase (SOD) and glutathione peroxidase (GPx). This study was intended to prove that the administration of tender coconut water 450 mL/day for 30 days can increase the antioxidant enzymatic and decrease lipid peroxidation. Methods Randomized Pre and Postest Control Group Design, a total of 76 gold mining workers were randomly selected divided into two groups of 38 each. The treatment group was given 450 ml of coconut water and the control one was given 450 mL of plain water for 30 days. The Blood test was conducted to evaluate of antioxidants including SOD, CAT, GPx and MDA as indicators of lipid peroxidation by Elisa method. Mann Whitney test was applied for data analysis. Results The mean level of SOD, CAT, GPx in the treatment group was higher than the that of control, while MDA was lower (SOD 337 ? 52.5 vs 267 ? 35.2 U/mL), CAT (2.0 ? 0.5 vs 1.9 ? 0.4 U/mL), GPx (79.9 ? 12.3 vs 68.8 ? 14.7 U/mL), MDA (3.6 ? 0.4 vs 4.5 ? 0.5 nmol / mL). The mean delta SOD, CAT, GPx of treatment group was larger than that of control, while MDA was smaller (SOD 75.4 ? 27.5 vs. -1.7 ? 8.2), CAT (0.23 ? 0.28 vs -0, 04 ? 0.25), GPx (10.40 ? 11.8 vs -1.55 ? 3.8), MDA (-0.95 ? 0.26 vs 0.1 ? 0.5). Conclusion the administration of tender coconut water 450 ml/day for 30 days can increase antioxidant SOD, CAT, GPx and decrease lipid peroxidation in subjects exposed to mercury.
Keywords: Mercury, ROS, SOD, CAT, GPx, MDA, tender coconut water
Edition: Volume 4 Issue 12, December 2015,
Pages: 517 - 524