Studies on Organogenesis (In vitro) in Boerhaavia. Diffusa L. A Medicinal Plant of Arid Region

Dr. Narpat Singh Deora

Abstract: Callus were raised on MS Medium supplemented with 2,4- D 1.00 mgl BAP 0.1 mgl from shoot segment and leaf of B. diffusa (Bhansali et al 1978, Biswas et al 2009) The callus shows luxuriant growth on same composition of medium for several multiplication cycle. organogenesis i.e., shoot induction were observed on MS medium containing BAP 1.0 mgl IAA 0.1 mgl.

Keywords: callus, organogenesis, shoot induction, medium, in vitro