International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064

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Analysis Study Research Paper | Microbial Genetics | Iraq | Volume 13 Issue 2, February 2024 | Rating: 5.4 / 10

Molecular Diagnosis of E. coli O157: H7 Isolated from Stored Food by RAPD - PCR

Ali H. Alwan | Mohammad Abdaljabbar | Saif Ali Mohammed

Abstract: Recently, and with the help of some newfangled techniques such as DNA\RNA testing (NAT), many microorganisms have been diagnosed accurately and quickly as we as their strains can be distinguished easily. Therefore, this study was prepared to design primers and some molecular laboratory tests for a specific gene that is found in bacteria Escherichia coli O157: H7, which has been obtained from some stored foods and these primers were designed using primer design software. In this study, a distinctive and unique region within the genetic material of a particular organism is selected as a template (i. e. sxt 1 gene for Escherichia coli O157: H7 was selected), then suitable primers were designed to prepare a RAPD - PCR to enable the amplification of this distinctive region, and then investigate it by migration using a gel electrophoresis technique. The purpose of using RAPD - PCR technique is the simplicity of its work and its low costs, in addition to its rather fast in work and its effectiveness in discovering bacteria that constantly form new strains such as Escherichia coli. The identification results were showed that the (NAT) analysis with RAPD ? PCR put by a specific, sensitive and rapid method for revelation the epidemiology of rapid food - borne infections.

Keywords: Nucleic Acid Testing (NAT) analysis, Escherichia coli O157: H7, RAPD, PCR

Edition: Volume 13 Issue 2, February 2024,

Pages: 656 - 660

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