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Comparative Studies | Hematology | India | Volume 11 Issue 9, September 2022

Correlation of Peripheral Smear with RBC and WBC Histogram in the Diagnosis of Anemia

Dr. Avani Patel | Dr. Ina Shah ^[2] | Dr. Hansa Goswami ^[15] | Dr. Vedant Shah ^[2]

Abstract: Introduction: Histograms produced by automated hematology analyzers are the graphical representation of numerical data of different cell populations. The automated hematology analyzer is alternative for traditional manual methods for measuring various hematological parameters as the initial screening method in most of the hospital nowadays. Aims: To correlate RBC and WBC histogram abnormalities with peripheral blood smear findings. Methods: Total of 500 blood samples of patients of the adult age group admitted in Civil Hospital, Ahmedabad were studied from August 2021 to December 2021 were taken. The study is conducted on hematology histograms for RBC generated by 5-part cell counter by Horiba Pentra XLRTM 80 and their degree of concurrence and non-concurrence with (Giemsa stained) peripheral smear findings respectively. Alterations in RBC, WBC histograms were analyzed with their respective peripheral smear findings. Results: In RBC histogram defects, the majority had a left shift followed by right shift and bimodal peak respectively. In WBC Histogram defects, WU defect, WL defect, Leucopenia, Leucocytosis. Conclusion: Histograms should be used as a screening method to pick up pathological samples. These samples should then be followed by a peripheral smear examination for confirmatory diagnosis.

Keywords: Automated hematological analyses, Histograms, RBC, Peripheral Blood Smear, WBC

Edition: Volume 11 Issue 9, September 2022,

Pages: 865 - 869

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