Development And Validation Of Hplc Method For The Simultaneous Estimation Of Loteprednol And Gatifloxacin

Abstract: A simple, rapid, precise and HPLC method for determination of Loteprednol and Gatifloxacin drugs in combined dosage form. The proposed HPLC method carried out on Hypersil BDS C18 (250 X 4.6mm, 5m) as stationary phase by using mobile phase consisting of 0.02M Phosphate buffer (pH 4.0 with orthophosphoric acid) acetonitrile (75 25). Mobile phase was pumped through chromatographic system at a flow rate of 1.0 ml/min. The UV detector was operated at 272nm. The retention time was found to be 3 min for Loteprednol And 7 min for Gatifloxacin. This optimize method was validated as recommended by ICH guidelines. The specificity studies shows that the analytic peaks were well resolved from the intermediates. The correlation coefficient were found 0.999 for Loteprednol and Gatifloxacin. The precision study showed that the percentage relative standard deviation was within the range of acceptable limits respectively. The limit of detection for Gatifloxacin is 0.248 and limit of quantification is 0.752 and for Lotprednol limit of detection is 0.486 and limit of quantification is 1.474.

Keywords: Loteprednol, Gatifloxacin, High performance liquid chromatography HPLC

Edition: Volume 2 Issue 5, May 2013,

Pages: 252 - 255