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Research Paper | Biology | Indonesia | Volume 8 Issue 6, June 2019

Ocimum sanctum: Phytochemical Screening and its Activity as Biopesticide against Musca domestica

Surahmaida | Umarudin

Abstract: A study of basil leaf extract (Ocimum sanctum) phytochemical screening and its potential as a botanical pesticides against houseflies (Musca domestica) has been carried out. The research method used was extracting basil leaves with maceration method using n-hexane solvents, phytochemical screening using chemical reagents and GCMS and its toxicity to houseflies by spray method. Variations in extract concentrations were 5 %, 10 %, 20 %, 30 % and 40 % and counted the number of dead flies at intervals of 5 minutes, 10 minutes and 15 minutes. The results of phytochemical screening with chemical reagents showed the presence of alkaloids, flavonoids and essential oils; and chemical compounds identified by GCMS, among others neral; 2, 4- (D2) menth-2-ene; 4, 5-Diclorotricyclo [5.3.1.1 (2, 6)] dodec-3-en-11-one; 1- (4-phenylcyclohexyl) -1-hexanone; ethyl linoleolate; 3 (5) - (4’-Chlorophenyl) -4-nitroso-5 (3) -phenylaminopyrazole; eicosane; 2- (Acetoxymethyl) -3- (methoxycarbonyl) biphenylene; 6H, 16H, 31H-5, 9: 15, 19-Dimethano-10, 14-metheno-26, 30-nitrilo-5H, 25H-dibenzo [b, s]. The toxicity test results showed that at a concentration of 10 % -40 %, n-hexane extract was able to kill Musca domestica by 100 % in the first 5 minutes of observation. It can be concluded that basil leaf extract can control houseflies.

Keywords: Bioactive compounds of n-hexane extract of Ocimum sanctum leaves, houseflies, mortality

Edition: Volume 8 Issue 6, June 2019,

Pages: 1547 - 1550