Extraction of Crude Acid Protease from Goat (Capra aegagrus) Abomasum

Tanalyna Hasna, Sri Mulyani, Umar Santoso, Yudi Pranoto

Abstract: The present study reports the extraction of crude acid protease from goat abomasum (CAPGA). The CAPGA was partially purified using different levels of ammonium sulphate precipitation and dialysis with dialysis sack MWCO 12 KDa. Protein concentration was determined by Bradford method using bovine serum albumin as the standard. The result shows that CAPGA extracted by ammonium sulphate precipitation of 75-100 % has highest protolithic activity. CAPGA activity and specific activity after being dialyzed was higher than before being dialyzed.

Keywords: ammonium sulphate, CAPGA, enzyme activity, extraction

How to Cite?: Tanalyna Hasna, Sri Mulyani, Umar Santoso, Yudi Pranoto, "Extraction of Crude Acid Protease from Goat (Capra aegagrus) Abomasum", Volume 8 Issue 5, May 2019, International Journal of Science and Research (IJSR), Pages: 1721-1722, https://www.ijsr.net/getabstract.php?paperid=ART20198133, DOI: https://dx.doi.org/10.21275/ART20198133