Determination of Bromate and Iodate from Bread and Flour by Ion Chromatography

Dr. Chetan Chavan, Chanakya Thaker, Chetan Chaudhari

Abstract: An accurate, simple, reproducible, and sensitive method for the determination of Bromate and Iodate was developed and validated. Bromate and Iodate were separated using a high capacity anion exchange column by isocratic elution with a flow rate of 1.0mL/min. Acidified Promethazine was added as post-column derivatization reagent (PCR). The mobile phase composition was 20mM Sodium Hydroxide and Ion chroamtographic PCR UV detection technique was carried out. The linearlity of method has been tested in the range of 0.03mg/l to 2.0mg/l for Bromate and 0.05 to 5.0mg/l for Iodate. Correlation cofficient (R2) of more than 0.999 was observed for both Bromate and Iodate. The method was shown excellent reproducible, linear, specific, sensitivity, rugged. The Limits of Detection and Quantification have been also established for Bromate as 0.01mg/l & 0.03mg/l and for Iodate as 0.03mg/l & 0.05mg/l respectively. Hence, the validated method is easy to adapt for regular analysis.

Keywords: Bread, Flour, Ion Chromatography, UV, Post Column Derivatization, Bromate, Iodate