Formulation, Method Development of Chemometric Assisted UV Spectrophotometric Method for the Estimation of Vitamins B1, B2 & B6

Devi Velmurugan

Abstract: Chemometric methods are the development of quantitative structure activity relationships or the Evaluation of analyticalchemical data. The data flood generated by modern analytical instrumentation is one reason, which analytical chemists in particular develop for applications of Chemometric methods. In this study, chemometric assisted UV spectrophotometric method was developed and validated for estimation of Vitamins B1, B2 & B6. It involves absorbance measurement at 264nm max of Thiamine Hydrochloride, 223 nm max of Riboflavin and 219nm max of Pyridoxine in Double distilled water. Linearity was obtained in the range of 2 10 g/ ml for the three drugs with correlation coefficient 0.9990 for three vitamins. The methods were validated as per International Conference on Harmonization Q2 (R1) (ICH) guidelines. These methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control as well as in process control of drugs and formulation.

Keywords: Vitamins B1, B2 & B6, UV spectroscopy, chemometric method, ICH guidelines